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KMID : 0382619850050020535
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Hanyang Journal of Medicine
1985 Volume.5 No. 2 p.535 ~ p.543
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The Effects of Cyclophosphamide on the Phosphatase Activities in the Mouse Liver
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Abstract
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Cyclophosphamide which is known as a widely used anticancerdrug is very effec-tive against a lymphosarcoma, leukemia and nonneoplasmic diseases such as rhematoid arthritis and lupus erythematosus. Cyclophosphamide acts on a DNA as a alkylating agent inhibiting the cell division and growth. And it is also known that metabolites of cyclophosphamide damages the liver cell.
The authors have, therefore, undertaken to pursue the effect of cyclophosphamide on the phosphatase activity in the liver. Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were administered daily with 200mg of cyclophosphamide per kilogram of body weight were divided in-to 7-day treatment group and 10-day treatment group. The specimens obtained from the liver were fixed with 10% formalin and sliced at 16 pm thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori´s method and activity of the adenosine triphosphatase was observed by the Wachstein-Meisel´s method for histochemical study.
The results were as follows.
1. Alkaline phosphatase activity of the hepatic lobule was moderately positive at the periportal and intermediate zone of the 7-da cyclophosphamide treatment group and trace activity of alkaline phosphatase was observed at the periportal and in-termediate zone of the 10-day cyclophosphamide treatment group.
2. Adenosine triphosphatase activity of the hepatic lobule was moderately positive at the periportal, central and intermediate zone of the 7-day cyclophosphamide treatment group and weakly positive at the central and periportal zone of the 10-day cyclophosphamide treatment group.
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KEYWORD
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